Assay of gentamicin in serum by high-pressure liquid chromatography.
نویسنده
چکیده
A procedure is described that uses high-pressure liquid chromatography for assay of gentamicin in serum. The technique involves extraction of gentamicin from serum by using a CM-Sephadex column and analysis by reverse-phase, ion-pair chromatography. Continuous-flow, post-column derivatization with o-phthaladehyde is used to form fluorescent products for detection. The possible scope of this method for analysis of other aminoglycoside antibiotics is illustrated by application to sisomicin and netilmicin.
منابع مشابه
Serum gentamicin assay by high-performance liquid chromatography.
We describe a high-performance liquid chromatographic method for the quantitative determination of gentamicin in serum. The antibiotic was separated from serum by passage through a silicic acid column, derivatized with o-phthalaldehyde, and eluted with ethanol. The derivatized gentamicin was then separated into all three of its major components by reversed-phase chromatography and quantified by...
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 11 4 شماره
صفحات -
تاریخ انتشار 1977